human colon cancer cell lines  (ATCC)

Journal: Discover Oncology

Article Title: High expression of LIMD2 predicts a poor prognosis and promotes migration of colon cancer cells

doi: 10.1007/s12672-025-03424-z

Figure Lengend Snippet: LIMD2 is upregulated in colon cancer tissues and cell lines. (A) LIMD2 expression was higher in colon cancer samples ( n = 10) compared to adjacent non-cancerous tissues ( n = 10). Scale: 50 μm and 500 μm. (B) LIMD2 mRNA expression was higher in the colon cancer tissues ( n = 10) than in the matched non-cancerous normal tissues ( n = 10). (C) RT-qPCR showed LIMD2 expression was higher in the colon cancer cell lines SW480, SW620, RKO, and HCT116 than in the normal colonic mucosal epithelial cell line FHC

Article Snippet: The human colonic mucosal epithelial cell line FHC and the human colon cancer cell lines SW480, RKO, SW620, HCT116 were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Expressing, Quantitative RT-PCR

Journal: PeerJ

Article Title: High matrix metalloproteinase-2 expression predicts poor prognosis of colon adenocarcinoma and is associated with PD-L1 expression and lymphocyte infiltration

doi: 10.7717/peerj.19550

Figure Lengend Snippet: (A) The correlation between MMP-2 expression and CD274 expression. R > 0.3, p < 0.05. (B) SW480 cells were transfected with MMP-2 siRNA, the level of MMP-2 and PD-L1 were detected by Western blot. (C) Relative expression of MMP-2, n = 3/group. (D) Relative expression of PD-L1, n = 3/group. ** p < 0.01, *** p < 0.001, ns , not significant ( p > 0.05).

Article Snippet: The human colon cancer cell line SW480 (Procell Life Science & Technology, Wuhan, China, batch of cells is CL-0223) and Caco-2 (Procell Life Science & Technology, Wuhan, China, batch of cells is CL-0050) were cultured in RPMI-1640 medium (HyClone, Logan, UT, USA), supplemented with 10% fetal bovine serum (FBS), 100 U/mL of penicillin, and 100 μg/mL streptomycin.

Techniques: Expressing, Transfection, Western Blot